ubc12 antibody Search Results


ube2m/ubc12 antibody (3c4)  (Bio-Techne corporation)
90
Bio-Techne corporation ube2m/ubc12 antibody (3c4)
Ube2m/Ubc12 Antibody (3c4), supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ubc12+antibody/UBE2M%2FUbc12+Antibody+(3C4)/bio-techne+corporation___h00009040-m01
Average 90 stars, based on 1 article reviews
ube2m/ubc12 antibody (3c4) - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
ubc12  (Novus Biologicals)
92
Novus Biologicals ubc12
Figure 5. Blockage of DCUN1D1 decreases global neddylation, ubiquitination and expression of neddylation components and shows preferential neddylation activity of cullin proteins in PCa. Western blot analysis of DU145 and DU145 DCUN1D1-KD cells. Protein extracts were obtained from the cells and subjected to Western blot analysis using (a) anti-ubiquitin, (b) anti-Nedd8. Blockage of DCUN1D1 decreased the expression of the neddylation pathway components including (c) the E1 NAE heterodimer APPB1 (top panel) and the neddylation-conjugating enzyme, <t>UBC12</t> (bottom panel) and (d) the cullin-associated proteins RBX1 (top panel) and CAND1 (bottom panel). Inhibition of DCUN1D1 showed preferential NEDD8 modification of the cullin family of proteins. Immunoblot analysis of DU145 and DU145 DCUN1D1 knockdown cell protein extracts using (e) anti-cullin 1, (f) anti-cullin 3, (g) anti-cullin 4A, (h) anti-cullin 4B and (i) anti-cullin 5. The GAPDH loading control was probed using the anti-GAPDH antibody. Experiments were independently repeated three times and a representative image of an independent experiment is represented.
Ubc12, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ubc12+antibody/UBE2M%2FUbc12+Antibody/pm37566052-113-73-74
Average 92 stars, based on 1 article reviews
ubc12 - by Bioz Stars, 2026-09
92/100 stars
  Buy from Supplier
anti ubc12  (Rockland Immunochemicals)
90
Rockland Immunochemicals anti ubc12
Figure 5. Blockage of DCUN1D1 decreases global neddylation, ubiquitination and expression of neddylation components and shows preferential neddylation activity of cullin proteins in PCa. Western blot analysis of DU145 and DU145 DCUN1D1-KD cells. Protein extracts were obtained from the cells and subjected to Western blot analysis using (a) anti-ubiquitin, (b) anti-Nedd8. Blockage of DCUN1D1 decreased the expression of the neddylation pathway components including (c) the E1 NAE heterodimer APPB1 (top panel) and the neddylation-conjugating enzyme, <t>UBC12</t> (bottom panel) and (d) the cullin-associated proteins RBX1 (top panel) and CAND1 (bottom panel). Inhibition of DCUN1D1 showed preferential NEDD8 modification of the cullin family of proteins. Immunoblot analysis of DU145 and DU145 DCUN1D1 knockdown cell protein extracts using (e) anti-cullin 1, (f) anti-cullin 3, (g) anti-cullin 4A, (h) anti-cullin 4B and (i) anti-cullin 5. The GAPDH loading control was probed using the anti-GAPDH antibody. Experiments were independently repeated three times and a representative image of an independent experiment is represented.
Anti Ubc12, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ubc12+antibody/UBC12+ANTIBODY/pmc04191716-86-43-44
Average 90 stars, based on 1 article reviews
anti ubc12 - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
ubc12  (Cell Signaling Technology Inc)
92
Cell Signaling Technology Inc ubc12
Figure 5. Blockage of DCUN1D1 decreases global neddylation, ubiquitination and expression of neddylation components and shows preferential neddylation activity of cullin proteins in PCa. Western blot analysis of DU145 and DU145 DCUN1D1-KD cells. Protein extracts were obtained from the cells and subjected to Western blot analysis using (a) anti-ubiquitin, (b) anti-Nedd8. Blockage of DCUN1D1 decreased the expression of the neddylation pathway components including (c) the E1 NAE heterodimer APPB1 (top panel) and the neddylation-conjugating enzyme, <t>UBC12</t> (bottom panel) and (d) the cullin-associated proteins RBX1 (top panel) and CAND1 (bottom panel). Inhibition of DCUN1D1 showed preferential NEDD8 modification of the cullin family of proteins. Immunoblot analysis of DU145 and DU145 DCUN1D1 knockdown cell protein extracts using (e) anti-cullin 1, (f) anti-cullin 3, (g) anti-cullin 4A, (h) anti-cullin 4B and (i) anti-cullin 5. The GAPDH loading control was probed using the anti-GAPDH antibody. Experiments were independently repeated three times and a representative image of an independent experiment is represented.
Ubc12, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ubc12+antibody/Ubc12+Antibody/10__1158_slash_1078___0432__ccr___15___2254-91-24-28
Average 92 stars, based on 1 article reviews
ubc12 - by Bioz Stars, 2026-09
92/100 stars
  Buy from Supplier
anti ubc12  (Proteintech)
94
Proteintech anti ubc12
Figure 5. Blockage of DCUN1D1 decreases global neddylation, ubiquitination and expression of neddylation components and shows preferential neddylation activity of cullin proteins in PCa. Western blot analysis of DU145 and DU145 DCUN1D1-KD cells. Protein extracts were obtained from the cells and subjected to Western blot analysis using (a) anti-ubiquitin, (b) anti-Nedd8. Blockage of DCUN1D1 decreased the expression of the neddylation pathway components including (c) the E1 NAE heterodimer APPB1 (top panel) and the neddylation-conjugating enzyme, <t>UBC12</t> (bottom panel) and (d) the cullin-associated proteins RBX1 (top panel) and CAND1 (bottom panel). Inhibition of DCUN1D1 showed preferential NEDD8 modification of the cullin family of proteins. Immunoblot analysis of DU145 and DU145 DCUN1D1 knockdown cell protein extracts using (e) anti-cullin 1, (f) anti-cullin 3, (g) anti-cullin 4A, (h) anti-cullin 4B and (i) anti-cullin 5. The GAPDH loading control was probed using the anti-GAPDH antibody. Experiments were independently repeated three times and a representative image of an independent experiment is represented.
Anti Ubc12, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ubc12+antibody/UBC12+Antibody/pmc05658359-309-20-23
Average 94 stars, based on 1 article reviews
anti ubc12 - by Bioz Stars, 2026-09
94/100 stars
  Buy from Supplier
anti ube2m antibody  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology anti ube2m antibody
Reagents and tools table
Anti Ube2m Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ubc12+antibody/Ubc12+Antibody/pmc11832924-39-0-3
Average 93 stars, based on 1 article reviews
anti ube2m antibody - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier
ubc12 antibody (fitc)  (biorbyt)
N/A
Rabbit polyclonal antibody against UBC12 conjugated to FITC Isotype Note: IgG Host Note: Rabbit Conjugation Note: FITC Reactivity Note: Human, Mouse, Rat Application Note: IF/ICC
   Buy from Supplier
ube2m/ubc12 antibody  (Novus Biologicals)
N/A
The UBE2M/Ubc12 Antibody from Novus is a UBE2M/Ubc12 antibody to UBE2M/Ubc12. This antibody reacts with Human. The UBE2M/Ubc12 antibody has been validated for the following applications: Western Blot, Immunohistochemistry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin.
   Buy from Supplier
ube2m/ubc12 antibody (1g9)  (Novus Biologicals)
N/A
The UBE2M/Ubc12 Antibody (1G9) from Novus is a UBE2M/Ubc12 antibody to UBE2M/Ubc12. This antibody reacts with Human. The UBE2M/Ubc12 antibody has been validated for the following applications: Western Blot, ELISA, Sandwich ELISA.
   Buy from Supplier
ube2m/ubc12 antibody (oti2d9) [dylight 594]  (Novus Biologicals)
N/A
The UBE2M/Ubc12 Antibody (OTI2D9) [DyLight 594] from Novus is a UBE2M/Ubc12 antibody to UBE2M/Ubc12. This antibody reacts with Human, Mouse, Rat, Canine, Monkey, Primate. The UBE2M/Ubc12 antibody has been validated for the following applications: Western
   Buy from Supplier
ube2m/ubc12 antibody (oti2d9) [janelia fluor® 549]  (Novus Biologicals)
N/A
The UBE2M/Ubc12 Antibody (OTI2D9) [Janelia Fluor® 549] from Novus is a UBE2M/Ubc12 antibody to UBE2M/Ubc12. This antibody reacts with Human, Mouse, Rat, Canine, Monkey, Primate. The UBE2M/Ubc12 antibody has been validated for the following applications:
   Buy from Supplier
ube2m/ubc12 antibody [alexa fluor® 405]  (Novus Biologicals)
N/A
The UBE2M/Ubc12 Antibody [Alexa Fluor® 405] from Novus is a UBE2M/Ubc12 antibody to UBE2M/Ubc12. This antibody reacts with Human. The UBE2M/Ubc12 antibody has been validated for the following applications: Western Blot.
   Buy from Supplier

Image Search Results


Figure 5. Blockage of DCUN1D1 decreases global neddylation, ubiquitination and expression of neddylation components and shows preferential neddylation activity of cullin proteins in PCa. Western blot analysis of DU145 and DU145 DCUN1D1-KD cells. Protein extracts were obtained from the cells and subjected to Western blot analysis using (a) anti-ubiquitin, (b) anti-Nedd8. Blockage of DCUN1D1 decreased the expression of the neddylation pathway components including (c) the E1 NAE heterodimer APPB1 (top panel) and the neddylation-conjugating enzyme, UBC12 (bottom panel) and (d) the cullin-associated proteins RBX1 (top panel) and CAND1 (bottom panel). Inhibition of DCUN1D1 showed preferential NEDD8 modification of the cullin family of proteins. Immunoblot analysis of DU145 and DU145 DCUN1D1 knockdown cell protein extracts using (e) anti-cullin 1, (f) anti-cullin 3, (g) anti-cullin 4A, (h) anti-cullin 4B and (i) anti-cullin 5. The GAPDH loading control was probed using the anti-GAPDH antibody. Experiments were independently repeated three times and a representative image of an independent experiment is represented.

Journal: Cells

Article Title: DCUN1D1 Is an Essential Regulator of Prostate Cancer Proliferation and Tumour Growth That Acts through Neddylation of Cullin 1, 3, 4A and 5 and Deregulation of Wnt/Catenin Pathway.

doi: 10.3390/cells12151973

Figure Lengend Snippet: Figure 5. Blockage of DCUN1D1 decreases global neddylation, ubiquitination and expression of neddylation components and shows preferential neddylation activity of cullin proteins in PCa. Western blot analysis of DU145 and DU145 DCUN1D1-KD cells. Protein extracts were obtained from the cells and subjected to Western blot analysis using (a) anti-ubiquitin, (b) anti-Nedd8. Blockage of DCUN1D1 decreased the expression of the neddylation pathway components including (c) the E1 NAE heterodimer APPB1 (top panel) and the neddylation-conjugating enzyme, UBC12 (bottom panel) and (d) the cullin-associated proteins RBX1 (top panel) and CAND1 (bottom panel). Inhibition of DCUN1D1 showed preferential NEDD8 modification of the cullin family of proteins. Immunoblot analysis of DU145 and DU145 DCUN1D1 knockdown cell protein extracts using (e) anti-cullin 1, (f) anti-cullin 3, (g) anti-cullin 4A, (h) anti-cullin 4B and (i) anti-cullin 5. The GAPDH loading control was probed using the anti-GAPDH antibody. Experiments were independently repeated three times and a representative image of an independent experiment is represented.

Article Snippet: The following primary antibodies were used: DCUN1D1 (Sigma HPA035911), Nedd8 (Cell Signaling Technology, Danver, MA, USA CST2745S), Ubiquitin (Cell Signaling Technology CST39365), cullin 1 (Cell Signaling Technology CST4995), cullin 2 (Novus Biologicals, Centennial, CO, USA NBP1-67535), cullin 3 (Cell Signaling Technology CST2759S), cullin 4A (Cell Signaling Technology CST2699S), cullin 4B (Bio-Rad VMA00360) Cells 2023, 12, 1973 6 of 22 cullin 5 (Novus Biologicals NBP1-22970), APPBP1 (Cell Signaling Technology CST14321), UBA3 (Novus Biologicals NBP2-48628), UBC12 (Novus Biologicals NBP1-31459), CAND1 (Cell Signaling Technology CST8759S), RBX1 (Novus Biologicals NBP2-20113), β-Catenin (Cell Signaling Technology CST4394S) phosphor-β-Catenin (Cell Signaling Technology CST2009S) and Lef1 (Santa Cruz sc-374522).

Techniques: Ubiquitin Proteomics, Expressing, Activity Assay, Western Blot, Inhibition, Knockdown, Control

Reagents and tools table

Journal: The EMBO Journal

Article Title: RHEB neddylation by the UBE2F-SAG axis enhances mTORC1 activity and aggravates liver tumorigenesis

doi: 10.1038/s44318-024-00353-5

Figure Lengend Snippet: Reagents and tools table

Article Snippet: Anti-UBE2M antibody , Santa Cruz , Cat #sc-100608; RRID: AB_2211030.

Techniques: Mutagenesis, Recombinant, CCK-8 Assay, Software